Determination of nimesulide in pharmaceutical and biological samples by a spectrophotometric method assisted with the partial least square method

Title
Determination of nimesulide in pharmaceutical and biological samples by a spectrophotometric method assisted with the partial least square method
Author(s)
Dr. (Mrs) Anupama Asthana
Issue Date
14-08-2012
Citation
-
Document Abstract
A simple and sensitive spectrophotometric method for the determination of nimesulide in bulk, in pharmaceutical dosage form, and in biological fluids was developed. The method is based on the reduction of the nitro group of nimesulide by zinc and hydrochloric acid followed by diazotization, and coupling with orcinol in basic medium to form a stable chromophore, which absorbs at 465 nm. The method showed a good linearity in the range 0.4–4.0 lg mL-1. Partial least square modeling as a powerful multivariate statistical tool is also applied, compiled, and compared for determination of nimesulide. The experimental matrix for the partial least square calibration method was designed with 24 samples. The cross-validation was used for selecting the number of factors. The root mean square error prediction (RMSEP) and the relative error of prediction (REP %) were 0.089 and 3.95, respectively. The developed method is free from the interference of common excipients used in pharmaceutical dosages. The method was also used for the determination of nimesulide in pharmaceutical dosages as well as in human serum and urine samples.
Language
English
Document Year
2012
Subject Name
Chemistry
Publisher Name
Res Chem Interned
Rights :