Document Abstract
A simple and sensitive spectrophotometric method for the determination
of nimesulide in bulk, in pharmaceutical dosage form, and in biological fluids was
developed. The method is based on the reduction of the nitro group of nimesulide by
zinc and hydrochloric acid followed by diazotization, and coupling with orcinol in
basic medium to form a stable chromophore, which absorbs at 465 nm. The method
showed a good linearity in the range 0.4–4.0 lg mL-1. Partial least square modeling
as a powerful multivariate statistical tool is also applied, compiled, and
compared for determination of nimesulide. The experimental matrix for the partial
least square calibration method was designed with 24 samples. The cross-validation
was used for selecting the number of factors. The root mean square error prediction
(RMSEP) and the relative error of prediction (REP %) were 0.089 and 3.95,
respectively. The developed method is free from the interference of common excipients
used in pharmaceutical dosages. The method was also used for the determination
of nimesulide in pharmaceutical dosages as well as in human serum and
urine samples.