Document Abstract
Two simple and sensitive spectrophotometric methods have been
developed for analysis of the antipsychotic drug olanzapine in pharmaceuticals.
Method A is based on liberation of iodine by reaction between the drug and
potassium iodate, followed by reaction with leuco crystal violet (LCV), the color of
oxidized LCV being measured at 598 nm. Method B is based on oxidation of
olanzapine with chloramine-T (CAT) in acidic medium, the unconsumed CAT
being determined with rhodamine B, measuring the absorbance at 550 nm. Calibration
graphs were linear over the ranges of 0.05–2.0 and 0.1–1.6 lg mL-1
olanzapine for method A and B, respectively. The molar absorptivity, Sandell’s
sensitivity, detection limit, and quantitation limit were found to be 1.59 9 105,
0.00132, 0.038, and 0.117, respectively, for method A and 0.953 9 105, 0.00221,
0.064, and 0.192, respectively, for method B. The optimum conditions and other
analytical parameters were evaluated. The proposed methods have been applied
successfully for analysis of olanzapine in pure form and its dosage forms, and no
interference was observed from common excipients present in pharmaceutical
formulations.